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KMID : 0923620120120060247
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Immune Network
2012 Volume.12 No. 6 p.247 ~ p.252
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Inhibition of Human Pancreatic Tumor Growth by Cytokine-Induced Killer Cells in Nude Mouse Xenograft Model
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Kim Ji-Sung
Park Yun-Soo
Kim Ju-Young
Kim Yong-Guk
Kim Yeon-Jin
Lee Hong-Kyung
Kim Hyung-Sook
Hong Jin-Tae
Kim Young-Soo
Han Sang-Bae
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Abstract
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Pancreatic cancer is the fourth commonest cause of cancer-related deaths in the world. However, no adequate therapy for pancreatic cancer has yet been found. In this study, the antitumor activity of cytokine-induced killer (CIK) cells against the human pancreatic cancer was evaluated in vitro and in vivo. Human peripheral blood mononuclear cells were cultured with IL-2-containing medium in anti-CD3 for 14 days. The resulting populations of CIK cells comprised 94% CD3＋, 4% CD3?CD56＋, 41% CD3＋CD56＋, 11% CD4＋, and 73% CD8+. This heterogeneous cell population was called cytokine-induced killer (CIK) cells. At an effector-target cell ratio of 100：1, CIK cells destroyed 51% of AsPC-1 human pancreatic cancer cells, as measured by the 51Cr-release assay. In addition, CIK cells at doses of 3 and 10 million cells per mouse inhibited 42% and 70% of AsPC-1 tumor growth in nude mouse xenograft assays, respectively. This study suggests that CIK cells may be used as an adoptive immunotherapy for pancreatic cancer patients.
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KEYWORD
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Pancreatic cancer, Adoptive immunotherapy, Cytokine-induced killer cells
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